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甘蓝OguCMS相关的花药优势表达转录因子BoBHLH1的克隆与表达分析 被引量：5

Cloning and Expression of an OguCMS-related Anther-preferential Transcription Factor in Brassica oleracea
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摘要 以甘蓝OguCMS花药中下调表达的EST序列为信息探针,克隆到一个与甘蓝OguCMS相关的bHLH家族转录因子,命名为BoBHLH1(GenBank登录号:GU390530),该基因编码102个氨基酸,具有典型的bHLH结构域,可识别顺式元件G-box序列,预测该蛋白定位在线粒体上。荧光定量RT-PCR表明,BoBHLH1是一个甘蓝花药中优势表达的基因,且在花药发育的早期和晚期均有一个表达高峰。研究结果为下一步分析其在甘蓝花药发育过程中的功能验证及OguCMS核质互作机理奠定了基础。 A new anther preferential bHLH transcription factor was cloned using a down-regulated expression EST sequence as a querying probe. The gene as BoBHLH1（GenBank accession number： GU390530）was designated. BoBHLH1 is a new member of bHLH transcription factors family related to OguCMS and contains an 309 nt of continuous complete open reading frame（ORF）encoding a polypeptide of 102 amino acids. BoBHLH1 is preferentially expressed in cabbage anther and have two expression peaks in early and late stages of anther development. The results provide new clues for further understanding of the nuclear-cytoplasmic interaction mechanism of OguCMS.
作者 刘海霞 康俊根 颉建明 简元才 丁云花
机构地区 北京市农林科学院蔬菜研究中心 甘肃农业大学农学院
出处 《园艺学报》 CAS CSCD 北大核心 2010年第12期1953-1960,共8页 Acta Horticulturae Sinica
基金 中国博士后科学基金项目(20080440325) ‘十一五’国家科技支撑计划项目(2008BADB1B02 2009BADB8B03) 北京市自然科学基金项目(6102012)
关键词 甘蓝 OGUCMS 基因克隆 bHLH转录因子 时空表达 cabbage OguCMS cloning bHLH transcription factor
分类号 S635 [农业科学—蔬菜学]
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参考文献22

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共引文献126

同被引文献138

  • 1余迪求,陈利钢,张利平.转录调控因子WRKY超级家族:起源、结构和功能[J].云南植物研究,2006,28(1):69-77. 被引量：22
  • 2严慧玲,方智远,刘玉梅,王永健,杨丽梅,庄木,张扬勇,孙培田.甘蓝显性雄性不育材料DGMS79-399-3不育性的遗传效应分析[J].园艺学报,2007,34(1):93-98. 被引量：20
  • 3向珣,曹家树,叶纨芝,王华新.白菜转录因子BcBHLHogu的克隆及其特征分析[J].中国农业科学,2007,40(8):1746-1752. 被引量：4
  • 4郑亚东,郭余龙,陈旭,李艳冬,欧建龙,李名扬.GhMADS3基因组成型表达对矮牵牛花形和花色的影响[J].园艺学报,2007,34(4):985-990. 被引量：12
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引证文献5

二级引证文献24

园艺学报
2010年 第12期

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