百合APX基因的克隆及转LlAPX提高拟南芥耐盐性

Molecular Cloning APX from Lilium longiforum and Overexpressing to Arabidopsis thaliana Enhanced Salt Tolerance

从铁炮百合‘白天堂’（Lilium longiforum ‘White Heaven’）组培苗叶片中克隆得到一个抗坏血酸过氧化物酶基因（APX）的cDNA序列,全长1074bp,推断其编码251个氨基酸。系统进化树分析表明APX在进化过程中保守性很高。通过构建过表达载体,转化拟南芥,其APX酶活性提高了4.7-7.8倍,AsA含量提高了1.5~2.3倍,其种子耐盐性提高。

An all-length cDNA of LlAPX gene was cloned from Lilium longiforum‘White Heaven’. The gene consisted of 1 074 bp encoding protein of 251 amino acids. Phylogenetic tree analysis showed that the APX had a highly conservative during evolution. We constructed a plant overexpression vector with LlAPX,and transgeniced to Arabidopsis thaliana. The APX activity in transgenic LlAPX Arabidopsis thaliana increased 4.7–7.8 fold,the AsA content increased 1.5–2.3 fold,and enhanced the salt tolerance in transgenic LlAPX Arabidopsis thaliana seeds.