那贺川野菊的离体保存

Studies on in Vitro Conservation of Chrysanthemum yoshinaganthum

以那贺川野菊为试材,研究了不同浓度蔗糖和多效唑（PP333）对试管苗离体保存的影响,并对保存材料再生后代的遗传稳定性进行了分析。结果表明：常温（23±2）℃、光照强度2000-3000lx、光照时间12h·d-1的培养条件下,在MS＋2.0mg·L-1KT＋0.1mg·L-1NAA＋6.5g·L-1琼脂培养基中,蔗糖浓度为15-30g·L-1时附加6-9mg·L-1PP333能保存试管苗360d以上,存活率达93.53%-100%,且恢复生长后试管苗长势良好,其再生后代的形态特征、过氧化物酶（POD）酶谱和ISSR-PCR扩增图谱与对照相比没有明显差异。

The effects of different concentrations of sucrose and PP333 on in vitro conservation of Chrysanthemum yoshinaganthum were investigated,and the genetic stability of the plants regenerated from the in vitro preserved germplasm was tested too. The results showed that plantlets can be conserved in vitro for 360 days in the medium MS ＋ KT 2.0 mg · L-1 ＋ NAA 0.1 mg · L-1 ＋ agar 6.5 g · L-1 supplemented with 15–30 g · L-1 sucrose and 6–9 g · L-1 PP333 under temperature（23 ± 2）℃, illumination intensity 2 000–3 000 lx and 12 h · d-1 photoperiod. The survival rate of plantlets was 93.53%–100%. The plantlets grew well after growth recovery. There were no significant differences in the morphology,peroxidase isoenzyme patterns and ISSR-PCR amplification profiles between the plants regenerated from in vitro preserved plants and non-preserved control,suggesting in vitro conservation didn’t affect the genetic stability of the germplasm.