猪瘟活疫苗(种毒)鉴别检验阳性血清制备

Preparation of antiserum for the identity test of classical swine fever live vaccine(seed virus)

为制备猪瘟活疫苗(种毒)鉴别检验阳性血清,本实验将4头非免疫健康猪进行猪瘟活疫苗基础免疫和猪瘟石门强毒株反复强化免疫后,采血分离血清,过滤、分装、冻干、熔封,获得4批产品,并进行了检验和验证。结果显示:物理性状、无菌检验、安全检验、均一性检验、支原体检验、真空度测定均合格;剩余水分均小于4%;特异性检验表明,口蹄疫病毒、猪伪狂犬病病毒、猪繁殖与呼吸综合征病毒、猪细小病毒、牛病毒性腹泻/粘膜病病毒抗体均为阴性;残余猪瘟病毒免疫荧光和套式RT-PCR核酸检测结果均为阴性;兔体中和效价分别为1∶800、1∶800、1∶800和1∶1 600;对猪瘟兔化弱毒种毒和5种猪瘟活疫苗的验证结果均良好。该4批血清可以用于猪瘟活疫苗(种毒)鉴别检验。

To prepare antiserum for the identity test of classical swine fever（CSF） live vaccine and its seed virus,healthy pigs were immunized with CSF live vaccine and boosted with CSFV virulent Shimen strain.Four lots of antiserum was prepared by filtration and lyophilization.All serum candidates passed the quality control tests.The specificity of the sera was tested and shown negative against foot and mouse disease virus,porcine pseudorabies virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus and bovine viral diarrhoea-mucosal disease virus.The CSFV residuals in the sera were also negative tested by IFA and nested RT-PCR.The neutralization titers of the sera were 1∶800,1∶800,1∶800 and 1∶1,600,respectively.The application of the sera was verified by testing seed virus of CSF vaccines and 5 vaccines.Therefore all 4 lots of sera could be used for the identity test of CSF live vaccine and its seed virus.