2-(2-苯并呋喃基)-2-咪唑啉对星形胶质细胞糖氧剥夺损伤的保护及抗脂质过氧化作用

The protective effect and antioxidative activity of 2-(2-benzonfuranyl)-2-imidazoline on oxygen-glucose deprivation of cultured rat cortical astrocytes

目的:探讨2-(2-苯并呋喃基)-2-咪唑啉(2-BFI)对星形胶质细胞糖氧剥夺(oxygen-glucosedeprivation,OGD)损伤的保护作用及脂质过氧化的影响。方法:制成星形胶质细胞糖氧剥夺损伤模型,分为正常对照组、OGD组、OGD+2-BFI干预组(浓度分别为0.75、1.5、3、6和12μg/mL),测定星形胶质细胞存活率,选出抗OGD损伤最佳浓度,以该浓度进一步观察2-BFI对脂质过氧化产物丙二醛(MDA)和还原型谷胱甘肽(GSH)含量的影响。结果:星形胶质细胞在OGD损伤后,不同浓度的2-BFI均能提高细胞存活率,其中1.5μg/mL的效果最佳;予1.5μg/mL 2-BFI干预OGD组能显著降低MDA含量,增加GSH含量。结论:2-BFI能保护星形胶质细胞OGD损伤,且有抑制脂质过氧化的作用。

Objective：To explore the neuroprotective effects and lipid peroxidation of 2-（2-benzonfuranyl）-2-imidazoline on oxygen-glucose deprivation of cultured rat cortical astrocytes.Methods：The model of oxygen-glucose deprivation of cultured rat cortical astrocytes was established.They were designed into three groups： normal control group,oxygen-glucose deprivation（OGD） group and oxygen-glucose deprivation＋2-BFI intervention group（the concentration of drug was 0.75,1.5,3,6 and 12μg/mL）,to determine the cell survival rate of astrocytes,the best drug concentration was chosen,then the best drug density was used to measure the content of malondialdehyde（MDA） and reduced glutathione（GSH）.Results：When astrocytes were deprived of oxygen and glucose,different concentration of 2-BFI could significantly raised the cell survival rate,and the effect of 1.5μg/mL was the best;1.5μg/mL 2-BFI intervention group could significantly reduced MDA level,and raised the content of reduced glutathione.Conclusion：2-BFI can effectively protect astrocyte injury after oxygen-glucose deprivation,and inhibit lipid peroxidation.