体内胶原网架生物学转归中碱性成纤维细胞生长因子的表达

Expression of basic fibroblast growth factor in biological transition of collagen lattice in vivo

背景：脱细胞真皮基质作为一种新型的真皮替代物,用于组织缺损的修复,但对于脱细胞真皮基质在长期生物学转归过程中碱性成纤维细胞生长因子的表达变化规律,国内外尚未见相关报道。目的：观察体内胶原网架长期生物学转归中碱性成纤维细胞生长因子的表达变化规律。方法：选用成年雄性Wistar大鼠21只用于制备脱细胞真皮基质。取84只大鼠,于脊柱左侧作一深至深筋膜表面、面积为2.5cm×2.5cm的创口,回植脱细胞真皮基质至成年雄性Wistar大鼠背部皮下,以对侧正常皮肤做对照。于术后3,5,7,10d,2,3,4,6,8,10周,4,6,8,10个月取材,采用免疫组织化学方法检测碱性成纤维细胞生长因子的表达。结果与结论：碱性成纤维细胞生长因子3d时开始出现阳性表达,并于10d达到峰值,4周后趋于稳定,8周后各组未见明显变化,与正常皮肤表达一致。提示在脱细胞真皮基质长期的生物学转归过程中,脱细胞真皮基质的改建首先从周边开始逐渐向中央迁移,并于6~8周后改建趋于稳定,被视为自身组织,并参与机体正常组织代谢过程。

BACKGROUND：As a new dermal substitute,acellular dermal matrix（ADM）has been extensively used in repair of tissue defects.But there are no reports about the expression of basic fibroblast growth factor（bFGF）during long-dated biological transition of ADM.OBJECTIVE：To discuss the rule of the bFGF expression in long-dated biological transition of collagen lattice in vivo.METHODS：A total of 21 Wistar rats were applied to prepare ADM.A wound at 2.5 cm×2.5 cm was made to deep fascia surface on left side of spine cord in 84 rats.ADM was transplanted to the back of Wistar rats,while contralateral normal skin served as the control.Samples were acquired at 3,5,7,10 days,2,3,4,6,8,10 weeks,4,6,8,10 months after operation.The bFGF expression was detected using immunohistochemical method.RESULTS AND CONCLUSION：Expression of bFGF came to appear in 3 days,and reached the peak in 10 days,then became stable in 4 weeks,after 8 weeks the expression of bFGF was the same with normal skin.In the course of long-dated biological transition of ADM,its rebuilding begins from the border of ADM gradually to the center;the rebuilding will become stable at 6-8 weeks after transplantation,then the ADM is considered to be autologous tissue and participates in the metabolic process of normal tissue.