应用猪主动脉脱细胞基质制备新型组织工程血管支架：生物相容性及力学性能评价(英文)

Acellular porcine aorta matrix as a novel tissue engineered vascular scaffold Biocompatibility and mechanical properties

背景:组织工程血管构建的关键依赖于理想的支架。猪血管作为组织工程血管构建材料已有广泛应用,但其较高的免疫原性及较差的力学强度限制了该材料作为组织工程支架的应用。目的:应用猪主动脉脱细胞基质制备一种新的具有良好机械性能及生物相容性的组织工程血管支架。方法:对猪主动脉进行脱细胞处理和热交联改性制备脱细胞血管基质支架,采用苏木精-伊红染色及生物力学分析评估其脱细胞效果及血管基质的力学性能。将人脐静脉血管内皮细胞接种于脱细胞血管基质支架中进行体外培养,评估其生物相容性。结果与结论:用1%的TritonX-100溶液处理猪主动脉84h可完全脱除血管细胞,同时不破坏血管基质结构;经真空下120℃热交联处理12h,脱细胞基质的拉伸断裂强度得到明显提高,达到1.70MPa。在该改性血管基质支架上接种人脐带静脉内皮细胞体外培养7d,扫描电镜显示内皮细胞呈现典型的血管内皮层状结构。表明猪主动脉经过脱细胞处理能够维持血管基质完整,冷冻干燥和真空热交联处理可有效提高其拉伸强度,且对血管内皮细胞具有良好的相容性。

BACKGROUND：The key of tissue engineered blood vessel research depends on the appropriate scaffolds.The porcine vascular tubes have frequently been used as candidates for tissue engineering vessel construction,but high immunogenicity and poor mechanical strength limit its application for tissue engineering scaffolds.OBJECTIVE：To prepare a novel tissue engineered vascular scaffold with good mechanical properties and biocompatibility using acellular porcine aorta matrix.METHODS：Porcine aorta was decellularized and modified by thermal cross-linking to improve the mechanical strength and biodegradation properties and to prepare acellular porcine aorta matrix scaffolds.Hematoxylin-eosin staining of histological sections and biomechanical tests were performed to assess the decellularization effects and the mechanical strength of the vascular matrix respectively.Vascular endothelial cells from human umbilical cord veins were isolated and seeded on the acellular matrix scaffolds and cultured in vitro.The biocompatibility was evaluated.RESULTS AND CONCLUSION：After the porcine aorta was treated by 1% Triton X-100 solution for 84 hours,the vessel was fully decellularized,and the architecture of matrix was well preserved.The acellular vascular matrix demonstrated improved biomechanical properties after modification by thermal cross-linking under vacuum at 120 °C for 12 hours.Its tensile strength reached 1.70 MPa.After 7 days of in vitro culture,the seeded endothelial cells formed a typical vascular endothelial layer structure on the surface of acellular matrix,as observed by scanning electron microscopy.Results proved that acellular vascular matrix of porcine aorta could maintain the morphology and structure of natural vessels,its mechanical strength could be greatly improved after successive freeze-drying and thermal cross-linking.A good compatibility between the acellular matrix and endothelial cells of umbilical vein is also achieved.