细胞外基质蛋白-1在原发性肝癌中的表达及其临床意义

Expression and clinical significance of extracellular matrix protein 1 in primary liver cancer

目的 检测细胞外基质蛋白-1（ECM1）在原发性肝癌中的表达,探讨其在肝癌转移中的临床意义.方法 收集2008年6月至2009年12月安徽医科大学附属省立医院手术切除的60例原发性肝癌患者的癌组织及癌旁组织标本和9例肝外伤患者的正常肝组织标本,采用免疫组织化学染色和Western blot法检测ECM1的表达,分析ECM1表达与肝癌临床病理特征之间的关系.率的比较采用x2检验和Fisher确切概率法,均数比较采用t检验.结果 免疫组织化学染色显示ECM1主要表达在细胞质中.肝癌组织中ECM1阳性表达率为73%,显著高于癌旁组织的20%和正常肝组织的22%（x2=34.286,7.044,P＜0.05）.ECM1的表达水平与肝癌有无转移和TNM分期有关（x2=5.455,4.275,P＜0.05）,而与患者性别、年龄、肿瘤大小、有无包膜、分化程度、血清AFP水平和HBsAg表达等无明显关系（x2=2.841,0.014,0.000,0.734,0.075,0.000,0.031,P＞0.05）.Western b1ot检测结果显示肝癌组织中ECM1相对含量为25.49±4.61,显著高于癌旁组织的3.00±0.37和正常肝组织的2.94±0.21（t=31.962,31.699,P＜0.05）.结论 ECM1在原发性肝癌组织中的表达高于癌旁和正常肝组织,且与肝癌有无转移和TNM分期有关,提示ECM1可能参与了肝癌的转移,可能是肝癌转移的预测指标之一.

Objective To detect the expression of extracellular matrix protein 1 （ ECM1 ） in primary liver cancer tissues, and explore its clinical significance in liver cancer metastasis. Methods Sixty cases of primary liver cancer tissues and adjacent tissues from 60 patients who were admitted to the Affiliated Provincial Hospital of Anhui Medical University from June 2008 to December 2009 were collected, and nine cases of normal liver tissues were collected from patients with liver trauma as control. The expression of ECM1 and the relationship between ECM1 and clinicopathological features of liver cancer were detected and analyzed using the immunohistochemistry and Western blot. All data were analyzed using the chi-square test, Fisher exact test and t test. Results ECM1 was mainly expressed in the cytoplasm of liver cells. The positive expression rate of ECM1 in liver cancer tissues was 73%, which was significantly higher than those in adjacent tissues （20%） and normal liver tissues （22%）（ x2 = 34.286, 7. 044, P 〈 0.05 ）. The expression of ECM1 was correlated with liver cancer metastasis and TNM stages （ x2 = 5. 455, 4.275, P 〈 0.05）, while not with sex, age, size, capsule and differentiation of the tumor,alpha fetoprotein level and the expression of hepatitis B surface antigen （ x2 = 2. 841, 0. 014, 0. 000, 0. 734,0.075, 0.000, 0.031, P〉0.05）. The result of Western blot indicated that the relative content of ECM1 in the liver cancer tissues was 25.49 ± 4.61, which was significantly higher than those in adjacent tissues （3.00 ±0.37） and normal liver tissues （2.94 ± 0.21 ） （ t = 31. 962, 31. 699, P 〈 0. 05 ）. Conclusions The expression of ECM1 in liver cancer tissues is higher than those in adjacent and normal liver tissues, and ECM1 expression is correlated with metastasis of liver cancer and TNM stages, which indicate that ECM1 may play a role in the metastasis of liver cancer, and it could be used as an indicator for liver cancer metastasis.