不同治法方药对激素性股骨头坏死的骨组织形态学影响

Effects of different Chinese drugs on bone histomorphology of hormone induced femoral head necrosis

目的:观察比较健脾化痰、活血通络与补肾壮骨、活血通络两种不同治法方药对激素性股骨头坏死形态学影响。方法:健康来航鸡64只,随机分为正常组,模型组,健脾化痰、活血通络组(简称健脾组)和补肾壮骨、活血通络组(简称补肾组),每组16只。分别于实验后8、16周取股骨头,采用HE和Masson染色方法观察其组织病理学形态。结果:与正常组相比,模型组髓腔内脂肪细胞面积增多(t=-12.9,P<0.01),股骨头内不成熟胶原面积明显增大(t=-2.0,P<0.05),骨小梁内空骨陷窝率显著增高(t=-3.7,P<0.05)。与模型组比较,2个给药组均能明显减少髓腔内脂肪细胞面积(F=26.8,13.5,P<0.01)和股骨头内不成熟胶原面积(F=4.6,P<0.01)。结论:健脾化痰、活血通络和补肾壮骨、活血通络两种不同治法方药均可减轻激素性股骨头坏死的组织形态病理学改变,前者发挥药效的时间相对较早。

Objective：To evaluate and compare the histopathology effects and mechanisms of the methods of ＂invigorating spleen to remove phlem promoting blood circulation to remove meridian obstruction＂ and ＂invigorate the kidney promoting blood circulation to remove meridian obstruction＂ preventing hormone induced femoral head necrosis in hens.Methods：Sixty four healthy hens were randomly divided into 4 groups：blank control group,model group,Jianpi group（with therapeutics of invigorating spleen to remove phlem）,Bushen group（with the effect of warming kidney for duresis）.All hens were injected intramuscularly with Medrat once a week for 16 weeks but normal saline in blank control group.Bilateral femoral heads were dissected on 8 weeks or 16 weeks.Paraffin tissue sections were prepared to detect histopathologic change with hematoxylin and eosin,or mason stainning.Results：Histological analysis showed that Huogu recipe I and Huogu recipeⅡcan promote osteogenesis and repair osteonecrosis,increase blood circulation of bone marrow,and inhibit pimelosis of bone marrow.Compared with blank control group,the areas of adipose cells increased significantly（t=-12.9,P0.01）,the area of immature collagen increased significantly（t=-2.0,P0.05） and the ratio of empty lacuna in medullary cavity（t=-3.7,P0.05）.Compared with model group,both the area of adipose cells and the ratio of empty lacuna decreased in Jianpi group and Bushen group（F= 26.8,13.5,P0.01）,so it was with the Bushen group immature collagen（F=4.6,P0.01）.Conclusion：Both the methods of ＂ invigorating spleen to remove phlem promoting blood circulation to remove meridian obstruction＂ and ＂invigorate the kidney promoting blood circulation to remove meridian obstruction ＂can prevent hormone induced femoral head necrosis.The time taking effect in the method of ＂invigorating spleen to remove phlem＂ was shorter.