大蒜素对Caco-2细胞胆固醇排出的影响

Effects of Allicin on Cholesterol Efflux in Caco-2 Intestinal Epithelial Cell

目的观察大蒜素对肠道细胞系Caco-2细胞胆固醇外排及胆固醇转运基因ABCG5/ABCG8表达的影响,探讨大蒜素对肠道细胞胆固醇转运的影响及机制。方法 Caco-2细胞随机分为对照组,胆固醇微胶粒组,胆固醇微胶粒加大蒜素组和大蒜素组4组。处理末,采用油红O染色观察细胞内脂滴,高效液相色谱法测细胞内胆固醇含量,免疫印迹法测ABCG5和ABCG8的蛋白表达。结果与对照组比较,胆固醇微胶粒增加Caco-2细胞内脂滴和胆固醇含量(P<0.05),而大蒜素减少胆固醇微胶粒处理后细胞内脂滴和胆固醇含量,增加细胞内胆固醇排出率(70.30%比20.92%,P<0.05)。大蒜素显著上调Caco-2细胞ABCG5和ABCG8的蛋白水平。结论大蒜素促进细胞内胆固醇排出,其机制可能与上调ABCG5和ABCG8表达相关。

Aim To explore the effects of allicin on cholesterol excretion and expression of ABCG5 and ABCG8 in Caco-2 cells.Methods Caco-2 cells were randomly divided into control group,cholesterol group,cholesterol plus allicin group,and allicin group.After treatment,oil O staining,high performance liquid chromatography and western blot were performed.Results Cholesterol treatment of Caco-2 cells showed increased intracellular lipid droplet and cholesterol contents compared with control group（P〈0.05）.However,Allicin significantly decreased lipid droplet and cholesterol contents in cholesterol-treated Caco-2 cells,increased efflux of cholesterol from the cells into the medium.Furthermore,allicin up-regulated the expression of ABCG5 and ABCG8 in Caco-2 cell.Conclusion These results suggest that allicin contributes to excretion of cholesterol from Caco-2 cell,which is related to up-regulation of ABCG5 and ABCG8 expression.