醛固酮对人脐静脉内皮细胞血管内皮生长因子表达的影响

The Impact of Aldosterone on Expression of Humbilical Vein Endothelial Cells Vascular Endothelial Growth Factor

目的醛固酮对人脐静脉内皮细胞增殖的影响及其机制。方法应用MTT法测试细胞的生长曲线,检测不同浓度的醛固酮对人脐静脉内皮细胞的增殖,流式细胞仪检测醛固酮对人脐静脉内皮细胞的凋亡,半定量RT-PCR及Western印迹分别检测人脐静脉内皮细胞的血管内皮生长因子mRNA水平及蛋白水平的表达。结果①MTT比色法显示,醛固酮对人脐静脉内皮细胞增殖具有抑制作用且呈浓度依赖性;②当醛固酮浓度增加到800μg/L后,其增殖抑制率不再升高,而此浓度时人脐静脉内皮细胞的凋亡率达到峰值的26.5%±3.3%;③经过醛固酮处理,人脐静脉内皮细胞的血管内皮生长因子mRNA及蛋白水平的表达量均显著下降。结论醛固酮通过降低人脐静脉内皮细胞的血管内皮生长因子表达抑制人脐静脉内皮细胞增殖并促进人脐静脉内皮细胞凋亡。

Aim To explore the inhibition of aldosterone（ALD）on proliferation of human umbilical vein endothelial cells（HUVEC）and its mechanism.Methods MTT was used to measure the proliferation.Flow cytometry assay（FCM）was applied for the detection of the apoptosis;Vascular Endothelial Growth Factor（VEGF）mRNA and VEGF protein were measured by semiquantitative RT-PCR and Western blot,respectively.Results ①Proliferation of HUVEC was inhibited by ALD in a dose-dependent manner,the cell viability of HUVEC decreased gradually.②However,when the concentration of ALD was above 800 μg/L,HUVEC were resistant to ALD.After the cells were incubated with ALD（800 μg/L）for 24 h.The rate of apoptosis in the HUVEC could reach 26.5%±3.3%.③The expression of VEGF mRNA and VEGF protein in HUVEC were decreased after it was incubated with ALD.Conclusion ALD can induce apoptosis and inhibit proliferation of HUVEC through changing the expression of VEGF.