糖基化终末产物对大鼠肾小球系膜细胞尾加压素Ⅱ及G蛋白偶联受体mRNA表达的影响

Effect of Advanced Glycation End Products on Expression of Urotensin Ⅱ and G-Protein-Couple Receptor mRNAs in Rat Mesangial Cells

目的观察不同浓度糖基化终末产物(Advanced glycation end products,AGEs)及AGEs作用不同时间对大鼠肾小球系膜细胞尾加压素Ⅱ(UrotensinⅡ,UⅡ)及G蛋白偶联受体(G-protein-couple receptor,GPR14)mRNA表达的影响。方法制备AGE-BSA,体外培养大鼠肾小球系膜细胞(Mesangial Cells,MC),加入不同浓度的AGE-BSA(终浓度分别为0、25、50、100和200mg/L),37℃孵育24h;加入100mg/L AGE-BSA,分别培养0、2、8、16和24h,以不含葡萄糖的BSA作为对照。收集细胞,采用RT-PCR检测各组MC UⅡ及GPR14mRNA的表达。结果 AGE-BSA各组MC UⅡ及GPR14mRNA的表达量均随AGEs浓度的增加而增加,50、100和200mg/L与0mg/L组比较,差异有统计学意义(P<0.05);100mg/L AGE-BSA各组MC UⅡ及GPR14mRNA的表达量随着作用时间的延长而增加,作用8、16、24h组与0h组比较,差异有统计学意义(P<0.05)。BSA组MC UⅡ及GPR14mRNA的表达量无明显增加(P>0.05)。结论 AGEs能上调大鼠MC UⅡ及GPR14mRNA的表达。

Objective To observe the effect of treatment with advanced glycation end products（AGEs）at various concentrations for various hours on the expression of urotensin Ⅱ（UⅡ）and G-protein-couple receptor（GPR14）mRNAs.Methods AGEBSA was prepared.Rat mesangial cells（MCs）were cultured in vitro,added with AGE-BSA to final concentrations of 0,25,50,100 and 200 mg/L respectively and incubated at 37℃ for 24 h,then added with 100 mg/L AGE-BSA and incubated for 0,2,8,16 and 24 h respectively,using glucose-free BSA as control.The cells were collected and determined for expressions of UⅡ and GPR14 mRNAs by RT-PCR.Results Both the expression levels of UⅡ and GPR14 mRNAs in the MCs treated with AGE-BSA increased with the increasing concentration of AGEs,which were significantly higher in the MCs treated with 50,100 and 200 mg/L AGE-BSA than those with 0 mg/L AGE-BSA（P 0.05）.However,in the MCs treated with 100 mg/L AGE-BSA,both the expression levels increased with the increasing time for incubation,which were significantly higher in the MCs incubated for 8,16 and 24 h than those for 0 h（P 0.05）.The expression levels of UⅡ and GPR14 mRNAs in the MCs treated with BSA showed no significant increase（P 0.05）.Conclusions AGEs up-regulated the expressions of UⅡ and GPR14 mRNAs in rat MCs.