电针对抑郁症大鼠海马组织AC-cAMP-PKA信号通路的影响

Effect of electroacupuncture on AC-cAMP-PKA signal transduction pathway in hippocampus of depression rats

目的探讨电针对抑郁症大鼠海马AC-cAMP-PKA信号通路的影响。方法 SD成年大鼠32只随机分为空白组、模型组、电针组(合谷+太冲)和药物组(盐酸氟西汀),每组8只。空白组进行正常饲养,不给予其他处理。模型组大鼠采用长期不可预见性温和刺激模型造成抑郁症;电针组大鼠造模后电针合谷穴和太冲穴,15min次/,1次/d左右两侧穴位交替进行,持续21d。药物组大鼠造模后进行氟西汀灌胃,1次/d,持续3w。应激后22d脱臼法处死大鼠,取海马组织,非标记底物法检测腺苷酸环化酶(AC)活性,用RIA法检测环磷腺苷(cAMP)含量及蛋白激酶A(PKA)活性。结果抑郁症模型大鼠海马中AC活性、cAMP含量、PKA活性均较空白组显著下降(均P<0.01);药物组和电针组大鼠海马AC活性、cAMP含量、PKA活性均较模型组显著升高(P<0.05或P<0.01)。结论抑郁症模型大鼠海马AC-cAMP-PKA信号通路下调,而电针可以通过上调AC-cAMP-PKA信号通路来发挥抗抑郁作用。

Objective To explore the effect of electroacupuncture on AC-cAMPPKA signal transduction pathway in hippocampus of depression rats.Methods Thirty-two adult Sprague-Dawley rats were divided randomly into control,model,medicine and electroacupuncture groups,8 rats in each group.Rats in control group was not given any other stimulation,and rats in model group were made depression by the chronic mild stress.Rats in medicine group were treated with fluoxetine hydrochloride for 3 weeks.In the electroacupuncture group,rats were given electroacupuncture stimulation for fifteen minutes,once per day for 3 week,following stress stimulation.At the 22th day,rats were killed by neckdislocating way,the hippocampus were taken and reserved in-70℃ for detecting. Adenylate cyclase（AC）was detected by non-labeled substrate;cyclic adenosine monophosphate（cAMP）and protein kinase A（PKA） were detected by RIA.Results AC activity,cAMP quantity and PKA activity in hippocampus of model group were decreased significantly than those of control group（P0.01）.AC activity,cAMP quantity and PKA activity in hippocampus of medicine group rats and the electroacupuncture group rats were increased significantly than those of the model group（P0.01）.Conclusions The AC-cAMP-PKA signal transduction pathway in hippocampus of depression rats is down-regulated,and electroacupuncture could up-regulate it to resist depression.