内阿片肽在“双固一通”针法保护缺血心肌中的作用

Role of endogenous opioid peptides in the protective effect of Shuangguyitong electroacupuncture on ischemic myocardial in rabbits

目的探讨延髓头端腹外侧区(rVLM)内阿片肽在"双固一通"针法电针对家兔心肌缺血损伤保护效应中的作用。方法本研究在"双固一通"针法对家兔急性心肌缺血保护作用的基础上,观察rVLM内微量注射纳洛酮后电针对急性心肌缺血家兔心电图及心肌细胞超微结构的影响。结果 rVLM内微量注射纳洛酮对正常家兔心肌功能和结构无明显影响,"双固一通"针法电针对缺血心肌具有一定的保护作用,能显著改善心肌缺血时心肌超微结构和心电图的变化(P<0.05),该作用可被纳洛酮所减弱(P<0.05),但不具有完全阻断作用。结论 "双固一通"针法对急性缺血心肌细胞具有保护效应,其作用与rVLM内源性阿片肽系统的介导有关。

Objective To detect the role of endogenous opioid peptides of rostral ventrolateral medulla（rVLM） in the protective effect of Shuangguyitong electroacupuncture on rabbit′s acute myocardial ischemia.Methods On the base of the protective effect of Shuangguyitong electroacupuncture on acute myocardial ischemia model in rabbits,electrocardiography（ECG） and myocardial ultrastructures of rabbit′s ischemic myocardial cell after microinjection naloxone into rVLM were observed.Results Microinjection naloxone into rVLM had no influence to normal rabbit′s myocardial function and myocardial ultrastructures and little influence to ischemic myocardial function.Shuangguyitong electroacupuncture had significant protective effect on acute ischemic myocardium in rabbits.Shuangguyitong electroacupuncture could markedly promote recovery of ST segment（P0.05）and obviously reduce the degree of ischemic myocardium injury.The protective effect could be weakened by microinjection with naloxone in rVLM（P0.05）,but couldn′t be completely blocked.Conclusions The effect of Shuangguyitong electroacupuncture in ameliorating acute myocardium ischemia in rabbits is mediated by opioid receptors in rVLM.