影响急性冠状动脉综合征患者基质金属蛋白酶-9相关因素的研究

Analysis of correlative factors to matrix metalloproteinase-9 in patients with acute coronary syndrome

目的:观察急性冠状动脉综合征(ACS)患者外周血清可溶性CD40配体(sCD40L)的水平、外周血单个核细胞(PBMCs)中核因子kappa B(NF-κB)的活性及两者与基质金属蛋白酶-9(MMP-9)之间的关系,探讨ACS预测和治疗的新思路。方法:采用酶联免疫吸附法测定所有入选对象外周血清sCD40L、MMP-9的浓度及PBMCs中NF-κB的活性。结果:ACS组外周血清sCD40L的浓度及PBMCs中NF-κB的活性均高于稳定型心绞痛组和正常对照组,均差异有统计学意义(P<0.05);ACS组血清sCD40L水平、PBMCs中NF-κB的活性与MMP-9呈显著正相关(P<0.01)。结论:ACS患者血清sCD40L水平及PBMCs中NF-κB的活性升高,可能是动脉粥样硬化斑块不稳定的标志之一;CD40L、NF-κB可能通过促使MMP-9的表达增加而促使ACS的发生。

Objective：To investigate serum soluble CD40L（sCD40L）level,the activity of nuclear factor kappa B（NF-κB）in PBMCs and their correlations with matrix metalloproteinase-9（MMP-9）in patients with ACS and to explore the potential predicting factor and the therapy for the ACS.Method：Serum sCD40L,MMP-9levels and the activity of NF-κB in PBMCs were determined by ELISA.Result：The concentration of serum sCD40Land the activity of NF-κB in acute coronary syndrome（ACS）group were higher than those instable angina pectoris patients and normal controls（both P〈0.05）.Serum sCD40Land the activity of NF-κB in ACS group were significantly correlated with MMP-9（both P〈0.01）.Conclusion：Serum sCD40Llevel and the activity of NF-κB in PBMCs were suggesting the possible relationship to the pathogenesis of ACS and they may serve as potential markers of plaque instability.sCD40Land NF-κB may promote the expression of MMP-9 and lead to the onset of ACS.