胆结石症患者纤维生成肝细胞的超微结构变化

Ultrastructural changes of hepatocyte fibrogenesis in cholelithiasis

目的探讨胆结石症患者肝细胞纤维生成的超微结构变化。方法收集10例胆结石症患者手术时提取的肝脏活体组织检查标本，在电子显微镜下观察其超微结构变化并作记录。结果6例肝细胞的细胞质内有局限性和弥漫性生长的胶原微纤维，甚至长入细胞核内。7例肝细胞出现较多的巨线粒体。其中的包涵物往往呈微纤维样，并能不断生长，穿越巨线粒体包膜。1例肝细胞中的微体能够生成胶原微纤维。1例肝细胞边缘出现类似成纤维细胞的囊泡。结论纤维化的肝细胞可成为肝纤维组织增生的一部分。

Objective To explore the ultrastructural changes of hepatocyte fibrogenesis in cholelithiasis in biliary tract. Methods 10 liver biopsies were taken from the patients suffered from gallstone and choledocholithiasis during surgical treatment and the ultrastructural changes were observed under electromicroscope. Results There were plentiful collagenous microfibrils （CMFs） grown within some hepatocytes. These CMFs distributed locally or diffusely in cytoplasm even extended into nucleus. In 7 cases numerous megamitochondrias appeared in several hepatocytes, the inclusions mimicking fibrils could be frequently seen and grew beyond the envelope. Furthermore, typical CMFs could be seen in the large microbodies, and several vesicular or cystic structures similar as fibroblast were presented in marginal areas of the hepatocytes. Conclusions We deduce that the fibrosed hepatocytes may be remained and take part in the hyperplasia of hepatic fibrous tissue.