miR-21在骨肉瘤中的表达及其临床意义

Expression of miR-21 in osteosarcoma and its clinical significance

[目的]探讨miR-21在骨肉瘤中的表达并分析其与临床病理之间的联系。[方法]本文通过实时定量PCR方法检测42例骨肉瘤组织(包括12例来自同一患者的原发骨肉瘤组织和转移肿瘤组织)和来自同一患者的邻近正常骨组织中miR-21的表达并评估miR-21的临床相关性。[结果]miR-21在所有的组织中都有表达,且在肿瘤组织中比在正常组织中有明显的高表达(P=0.013),此外,miR-21在转移的肿瘤组织中比在同一样本中的原发肿瘤组织中高表达(P=0.002),另外,miR-21高表达的患者有着显著更差预后(P=0.016)。[结论]miR-21高表达潜在的导致转移,其可以作为骨肉瘤患者预后的一个指标。

[Objective]To explore the expression of miR-21 in osteosarcoma and the correlation between miR-21 and clinicopathological characteristics.[Method]We analyzed the level of miR-21 expression in osteosarcoma tissues,matched it to the normal bone tissues from 42 patients（including 12 sets of matched primary or metastatic tumor samples from the same patients） by real-time RT-PCR and evaluated the clinical relevance of miR-21.[Result]Totally 38 of 42（90.5%） showed a higher expression level of miR-21 in tumor tissues than in matched normal tissues.Moreover,analysis of paired primary and metastatic osteosarcoma samples from 12 patients showed an increase in miR-21 expression levels in metastases.Besides,higher expression of miR-21 had a significant correlation with reduced survival.[Conclusion]Our results suggest that overexpression of miR-21 potentially contributes to human osteosarcoma metastasis and acts as one of prognostic factors.