摘要
应用超高效液相色谱-质谱联用技术(UHPLC-MS)与气相色谱-质谱联用技术(GC-MS),对环保水性印油中的主要成分(主要颜料与挥发性物质)进行了定性分析。通过超声提取与离心对样品进行预处理后,在ZORBAXEclipse Plus Phenyl-Hexyl(50mm×4.6mm,1.8μm)液相色谱柱,15mmol/L乙酸铵水溶液-乙腈为流动相,在UHPLC-MS负离子电喷雾电离条件下以选择离子监测模式定性分析染料及颜料;采用HP-INNOWAX(30m×0.25mm,0.25μm)气相色谱柱进行GC-MS全扫描,定性分析挥发性物质。研究确认水性印油中的主要颜料成分是酸性红R、水溶曙红Y与颜料红112,主要挥发性物质是甘油、1,2-丙二醇等。本方法快速、准确,可以满足物证鉴定工作中对印记的检测需要,有助于法庭科学中对印油印记的种类区分。
Ultra high performance liquid chromatography-mass spectrometry(UHPLC-MS) technology and gas chromatography-mass spectrometry(GC-MS) technology were used to qualitatively analyze the major components in water based stamp pad inks including major colorants and volatile components.After the samples were supersonically extracted and then centrifuged,UHPLC-MS was used to separate and identify the major colorants.A ZORBAX Eclipse Plus Phenyl-Hexyl(50 mm×4.6 mm,1.8 μm) column and 15 mmol/L ammonium acetate-acetonitrile were utilized for the separation and negative selected ion monitoring mode(SIM) was set for the MS analysis.An HP-INNOWAX(30 m×0.25 mm,0.25 μm) column was employed in the GC-MS analysis with the full-scan mode to determine the volatiles.This study demonstrated that the major colorants in the inks and their imprints were Acid Red R,Eosin Y and Pigment Red 112;and the major volatiles were glycerol,1,2-propanediol,etc.The method is rapid and accurate.It also demonstrates that the method can meet the requirements for imprint determination in material evidence identification.The work provides a reliable tool for the categorization research in the forensic sciences.
出处
《色谱》
CAS
CSCD
北大核心
2010年第12期1132-1136,共5页
Chinese Journal of Chromatography
基金
中央级公益性科研院所基本科研业务费专项资金重点项目(No.2009JB008)
关键词
超高效液相色谱-质谱
气相色谱-质谱
水性印油
印记
ultra high performance liquid chromatography-mass spectrometry(UHPLC-MS)
gas chromatography-mass spectrometry(GC-MS)
water based stamp pad inks
imprints