摘要
实验采用农杆菌LBA1100介导转化马铃薯品系的春薯3号,优化了马铃薯转化的几个影响因素:①外植体采用叶片诱导比节间诱导有利于愈伤组织的形成;②农杆菌侵染时间为8-10分钟时转化率最高;③菌液浓度OD600约为0.2-0.4之间时转化率最高.
An efficient and rapid Agrobacterium-mediated transformation procedure has been established in potato(Chunshu-3): ① Potato Leaves are better than potato internode for callus induction.② The optimal time duration for inoculation of explants in Agrobacteria culture was 8-10 minutes.③ Optimal concentration of Agrobacteria for inoculation was OD_(600)=0.2-0.4.
出处
《山东教育学院学报》
2010年第6期46-48,共3页
Journal of Shandong Education Institute
关键词
马铃薯
农杆菌
转化
优化体系
Potato
Agrobacterium tumefaciens
Transformation
The efficient