酶法水解血球蛋白工艺及其参数研究

On Technique Conditions and Parameter of Enzymatic Hydrolysis Porcine Hemoglobin

本试验通过测定酶解的水解率,选择水解猪血血球蛋白的最优蛋白酶,并确定酶的最佳水解条件(包括底物浓度、酶添加比例、pH值、温度、反应时间以及沉淀血红素的pH值)。试验结果表明,最佳水解条件为底物浓度10%、酶添加比例8‰、pH7.5、水解温度53℃、水解时间为10h。

According to mensuration of enzymatic hydrolysis percentage, the optimization protease was selected and the optimum hydrolysis condition was determined. The hydrolysis condition include the substrate concentration, proportion of enzymes, pH, hydrolysis temperature and hydrolysis times. The result shows that the optimum hydrolysis conditions of enzymatic hydrolysis porcine hemoglobin are as follow follows ： the substrate concentration 10%, proportion of enzymes 8‰, pH 7.5, hydrolysis temperature 53℃, hydrolysis times 10h.