甲氨基阿维菌素苯甲酸盐对镇海林蛙蝌蚪的遗传毒性

Genotoxicity of emamectin benzoate on Rana zhenhaiensis tadpoles

以镇海林蛙(Rana zhenhaiensis)蝌蚪为研究对象,用碱性单细胞凝胶电泳方法(慧星试验,SCGE)检测不同质量浓度的甲氨基阿维菌素苯甲酸盐(甲维盐)对其的遗传毒性.结果表明,在实验室条件下,随着农药质量浓度的增加,蝌蚪红细胞的损伤率(拖尾率)及DNA损伤程度随之增加;在浓度为3×10-2 mg/L的甲维盐溶液中处理24 h后,与对照组相比,镇海林蛙蝌蚪红细胞的损伤率及DNA损伤程度有显著的提高;甲维盐的剂量与蝌蚪红细胞损伤率及DNA损伤程度呈显著的线性相关关系.研究表明,甲维盐对两栖动物蝌蚪具有一定的遗传毒性作用,在检测环境污染物对两栖类的遗传毒性方面,慧星试验是一种合适的方法.

Genotoxicity of emamectin benzoate on erythrocytes of Rana zhenhaiensis tadpoles was investigated by the alkaline single-cell gel electrophoresis assay or comet assay.Tadpoles treated for 24 h and 48 h in laboratory with different concentrations（3×10-2,4×10-2,5×10-2,6×10-2 mg/L） of the test agent had significant dose-responsive increases at the level of DNA damage.The concentration of 3×10-2 emamectin benzoate resulted in significant increases in DNA damage in the tadpoles.There were linear correlations between the damage frequency,DNA damage level and the concentrations of the test substance.The results showed that the agricultural chemical produced DNA damage in amphibians,and that comet assay might be a useful tool for measuring DNA damage in tadpoles exposed in the field.