模拟消化道出血时大便潜血试验金标法的研究

Research on colloidal gold method of fecal occult blood testing in simulating gastrointestinal hemorrhage

目的探讨低浓度血红蛋白(Hb)液被生理浓度的酸、胃蛋白酶和胰蛋白酶破坏和消化作用后金标法大便潜血试验的情况。方法 (1)配制一定浓度梯度的血红蛋白液,用艾康生物技术有限公司提供的金标法大便OB试纸条验证其灵敏度。(2)配制一定浓度梯度的血红蛋白液,分别与生理浓度的酸、胃蛋白酶和胰蛋白酶孵育不同时间后,用金标法大便OB试纸条检测。结果 (1)本试验的金标法OB试纸条的灵敏度为0.1μg/mL。(2)不同浓度的低浓度的血红蛋白液在孵育30 min后基本上全部被胃蛋白酶消化,在孵育1.5 h后基本上全部被胰蛋白酶消化。(3)生理浓度范围内的不同浓度的胰蛋白酶对同一低浓度的血红蛋白液的消化时间基本上一致,且经不同浓度的胰蛋白酶消化后,金标法潜血试验结果的阳性程度呈渐降过程,最后阴性。结论 (1)本试验所用的艾康生物技术有限公司的金标法大便OB试纸条的灵敏度为0.1μg/mL。(2)在模拟消化道出血,特别是少量出血时,不同浓度的低浓度血红蛋白液在经酸、胃蛋白酶和胰蛋白酶破坏和消化降解后可能导致金标法大便潜血试验假阴性。

Objective To study the result of colloidal gold method（GM） of fecal occult blood testing with hypo-concentrations of Hb which was digested by physiological concentration of acid,pepsin and trypsin.Methods（1）To prepare the certain concentration gradient of Hb,test and confirm the sensitivity of colloidal gold OB test strips.（2）The certain concentration gradient of Hb was mixed with physiological concentration of acid,pepsin and trypsin respectively,tested with colloidal gold of OB test strips after incubation at different times.Results（1）The sensitivity of colloidal gold of OB test strips was 0.1 μg/mL.（2）After incubated,different concentrations of Hb were digested fully by pepsin at 30 min and by trypsin at 1.5h.（3）The physiological concentration range of different concentrations of trypsin had the same time on digesting the same level of hypo-concentrations of Hb and the result of the positive extent was decreasing gradually and was negative finally.Conclusion（1）The sensitivity of colloidal gold of OB test strips produced by Aicon Bio-Technology Co.is 0.1 μg/mL.（2）The different concentrations of hypo-concentrations Hb are digested out respectively by the physiological concentration range of acid,pepsin and trypsin in imulating gastrointestinal hemorrhage,which causes the test result of colloidal gold method of fecal occult blood testing negative.