HPLC测定大黄虫丸中芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚的含量

Determination of Aloe-emodin,Rhein,Emodin,Chrysophanol and Physcion in Dahuang Zhechong Pills by HPLC

目的:建立HPLC同时测定大黄虫丸中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的方法。方法:色谱柱为Shimadzu VP-ODS柱(4.6mm×250mm,5μm),流动相为乙腈-0.1%磷酸梯度洗脱,流速为1.0mL·min-1,检测波长为254nm,柱温为30℃。结果:芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚5种成分分别在0.042～0.840μg(r=0.9996)、0.168～3.352μg(r=0.9998)、0.084～1.680μg(r=0.9997)、0.093～1.852μg(r=0.9999)和0.174～3.488μg(r=0.9994)呈良好线性关系,平均回收率分别为98.87%(RSD=1.43%)、98.63%(RSD=0.64%)、97.80%(RSD=1.46%)、97.29%(RSD=0.97%)和98.45%(RSD=0.88%)。结论:本法简便、快速、准确,可用于大黄虫丸的质量控制。

Objective： To develop a HPLC method for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Dahuang Zhechong Pills. Methods： The sample was separated on a Shimadzu VP-ODS（4. 6 mm ×250 mm, 5 um） with acetonitrile-0. 1% phosphoric acid in gradient elution. The flow rate was 1.0 mL. min-1, the detection wavelength was 254 nm and the column temperature was 30 ℃. Results： Aloe-emodin, rhein, emodin, chrysophanol and physcion had good linear relationship in the range of 0. 042 × 0. 840 ug （r = 0. 999 6） ,0. 168 -3. 352 ug（r = 0. 999 8） ,0. 084 - 1. 680 ug（r =0. 999 7） ,0. 093 - 1. 852 ug（r =0. 999 9）and 0. 174 -3. 488 ug（r =0. 999 4）. Their average recoveries were 98. 87 % （RSD = 1.43 %, n =6）, 98. 63 % （RSD = 0.64 %, n=6）, 97.80 %（RSD=1.46 %, n=6）, 97.29 %（RSD=0.97 %, n=6）, and 98.45 %（RSD= 0. 88 %, n = 6）, respectively. Conclusion： The method is rapid, simple, accurate, and applicable for the quality control of Dahuang Zhechong pills.