尿碘测定方法的改进

An Improved Method for the Measurement of Iodine in Urine

根据国标法尿碘砷铈催化分光光度法原理，在保持反应温度和试剂浓度不变的条件下，将Ｃｅ４＋在碘催化作用下变成Ｃｅ３＋的反应时间由原来的１５ｍｉｎ改为１０ｍｉｎ，使得尿碘测定的线性范围由原来的０～３００μｇ／Ｌ拓宽至０～４５０μｇ／Ｌ，而试样体积（０．２ｍＬ）和方法的最低检出浓度（６μｇ／Ｌ）仍与国标法一样．结果表明：改进的尿碘测定法精密度、准确度和重现性均良好，所有实验结果的变异系数不超过５．５％，平均回收率为１０１．３％，经符号检验，其方法与国标法的测定结果之间无显著差异（显著水平为１０％）．可缩短实验时间。

The method for the measurement of iodine in urine has been improved. The time for the reduction of Ce 4+ to Ce 3+ by H 3AsO 3 is shorted from 15 to 10 min under the constant conditions of temperature, reagent and concertration. The linear range of this method for the determination of iodine in urine has been widened from 300 μg/L to 450 μg/L. The CV is less than 6% and the average recovery ratio is 101.3%. There is no evidental difference between the results determined by this improved method and those by the GB/T method.