摘要
采用GC和GC-FTIR法,研究了N2和N2O对固氮酶催化C2D2还原活性及产物二氘代乙烯的反式-/顺式-异构体比值的影响,结果表明,N2和N2O均抑制C2D2的还原作用,并引起反式-/顺式-1,2-二氘代乙烯比值的增加.这一变化规律,与根据C2H2还原被N2和N2O抑制只发生在固氮酶铁钼辅基(FeMoco)的笼内的假设而近似计算的结果相一致.实验结果和理论计算之间的一致性支持N2和N2O结合在FeMoco的笼内。
The effects of N 2 and N 2O on C 2D 2 reduction activity and ratio of trans /cis 1,2 dideutroethylene in products catalyzed by nitrogenase have been studied using GC and GC FTIR measurements. The results showed that N 2 or N 2O can inhibit the C 2D 2 reduction,and result in the increase of the trans /cis ratio in CHD=CHD. The changes of the trans /cis ratio in 1,2 dideutroethylene are in line with the results from approximate calculations based on the hypothesis that the inhibition of C 2H 2 reduction by N 2 or N 2O takes place practically only inside the cage.The consistency between the experimental results and the approximate calculation supports the suggestions that N 2 and N 2O bound to inside the cage and mainly inhibit the C 2D 2 reduction inside the cage.
出处
《厦门大学学报(自然科学版)》
CAS
CSCD
北大核心
1999年第4期611-616,共6页
Journal of Xiamen University:Natural Science
基金
国家"八.五"攀登课题资助项目
国家自然科学基金
关键词
固氮酶
钼铁蛋白
氮
结合位
一氧化二氮
Nitrogenase
C 2D 2 reduction, Trans /cis 1,2 dideutroethylene, Binding sites of N 2 and N 2O