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叶尔羌高原鳅肝胰脏总RNA提取方法的比较 被引量：1

Comparison of the Extracting Method of Total RNA in Hepatopancreas of Triplophysa (Hedinichthys) Yarkandensis
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摘要 比较利用TRIZOL法和用TaKaRa的RNAiso Plus法,两种方法提取叶尔羌高原鳅肝胰脏总RNA的可行性,通过琼脂糖凝胶电泳、紫外分光光度计检测及一步法RT-PCR验证,对提取所得RNA的完整性、纯度及浓度进行分析,试验结果表明:TRIZOL法获得的叶尔羌高原鳅总RNA纯度高,完整性好,可以继续进行分子克隆试验。 Compare with the feasibility of the TRIZOL extraction method and the TaKaRa＇s RNAiso Plus extraction method for the extraction of the total RNA in hepatopancreas from Yarkant Triplophysa.The integrality,purity and concentration of the total RNA was analyzed by using the agarose gel electrophoresis,UV spectrophotometer and One-step RT-PCR validation.The results showed that the TRIZOL method gained high purity and excellent integrity of total RNA of Yarkant Triplophysa,and can be used for the continued molecular cloning experiment.
作者 徐丽君 潘辉 贺艳艳 刘书东 左文斌 李莲瑞
机构地区 塔里木大学生命科学学院 塔里木大学动物科学学院
出处 《塔里木大学学报》 2010年第4期18-21,共4页 Journal of Tarim University
基金 兵团博士基金(2009JC18)
关键词 叶尔羌高原鳅 TRIZOL法 RNAisoPlus提取法 总RNA 提取 纯度 Yarkand Triplophysa TRIZOL method RNAiso Plus extraction total RNA extract purity
分类号 Q959.4 [生物学—动物学]
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  • 1卢强,丰培金,李莲瑞,付宝权,刘明远.正常鲤外周血白细胞cDNA文库的构建[J].水产学报,2004,28(5):585-588. 被引量：17
  • 2卢强,丰培金,李莲瑞,付宝权,刘明远.有丝分裂原刺激的鲤鱼外周血白细胞cDNA文库的构建[J].中国兽医学报,2004,24(6):568-570. 被引量：17
  • 3[1]Day,F.On the fishes of Yarkand.Proc.Zool.Soc.London,1876,(53):781～807.
  • 4武云飞 吴翠珍.青藏高原鱼类[M].成都:四川科学技术出版社,1991..
  • 5韩文瑜.现代分子病原细菌学[M].长春:吉林人民出版社,2003.158-178.
  • 6CHOPRA A K,HOUSTON C W,PETERSON J W,et al.Cloning,expression,and sequence analysis of a cytolytic enterotoxin gene from Aeromonas hydrophila [J].Can J Microbiol,1993,39:513～523.
  • 7DONTA S T,HADDOW A D.Cytotoxic activity of Aeromonas hydrophila [J].Infect Immun,1978,21:989～993.
  • 8WILMSEN H U.Aerolysin from Aeromonas hydrophila,forms voltage-gated channel in planar lipid bilayers[J].S Member Biol,1990,115 (1) : 71～81.
  • 9HENNING S.Antibiotic resistance in aquaculture[J].Acta Vet,1999,92(Suppl):29～36.
  • 10MUDER R R,BRENNEN C,GOETZ M A,et al.Association with prior fluoroquinolone therapy of widespread ciprofloxacin resistance among gram-negative isolates in a veterans affairs medical center[J].Antimicrob Agents Chemother,1999,35: 256～258.

共引文献38

同被引文献10

  • 1Michael Zasloff. Antimicrobial peptides of muhicellular organisms [ J ]. Nature,2002,415 (24) :389 - 395.
  • 2Ivana De Domenico, Elizabeta Nemeth, Jenifer M. Nel- son, et al. The Hepcidin - Binding Site on Ferroportin Is Evolutionarily Conserved [ J ]. Cell Metabolism ,2008, 8 (2) : 146 - 156.
  • 3Chanprapa Imjongjirak, Piti Amparyup , Anchalee Tas- sanakajon . Molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (ALF) from the mud crab [ J ]. Scylla paramamosain Fish or Shellfish Immunology, 2011, 30 (1) : 58 -66.
  • 4Margitta Dathe, Heike Nikolenko, Jana Meyer, et al. Optimization of the antimicrobial activity of magainin peptides by modification of charge [ J ]. FEBS Letters, 2001,501 ( 2 - 3 ) : 146 - 150.
  • 5Hunter H N, Fulton D B, Ganz T, et al. The solution structure of human hepcidin, a peptide hormone with antimicmbial activity that is involved in iron uptake and hereditary hemochromatosis [ J ]. BIO Chem, 2002, 277 (40) : 37597 - 37603.
  • 6Weinstein D A, Roy C N, Fleming M D, et al. Inappro-priate expression of hepcidin is associated with iron re-fractory anemia: implication for the anemia of chronic disease[ J]. Blood,2002, 100(10) :3776 - 3781.
  • 7Yin ZhiXin, He Wei, Chen WeiJian,et al. Cloning, expression and antimicrobial activity of an antimicrobial peptide, epinecidin - 1, from the orange - spotted group-er, Epinephelus coioides [ J ]. Aquaculture, 2006,253 ( 1 -4) :204 -211.
  • 8Piraee M, Ving L C. Use of degenerate primers and touchdown PCR to amplify a halogenase gene flagment from Streptomyces venezuelae ISP5230 [ J ]. Journal of Industrial Microbiology & Biotechnology ,2002,29 ( 1 ) : 1-5.
  • 9J萨姆布鲁克著 金冬雁 黎孟枫译.分子克隆实验指南(第二版)[M].北京：科学出版社,1992.464-469.
  • 10张艳萍,郭大玮,马莉莉.降落PCR扩增人类常染色体STR D15S128[J].中国现代医生,2010,48(2):38-39. 被引量：1

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塔里木大学学报
2010年 第4期

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