Mutations of mitochondrion DNA in mouse tumors

Objective： To ascertain the variations of mitochondrion DNA （mtDNA） in mouse tumors and to inquire into the relationship between mutations of mtDNA and carcinogenesis Methods： The variations of D-loop, ND3 and tRNA^Met＋Glu＋Ile gene fragments of mtDNA from six tumor cell lines of mice were analyzed by PCR technology with restriction fragment length polymorphism analysis （polymerase chain reaction-restriction fragment length polymorphism, PCR-RFLP） and single strand conformation polymorphism analysis （SSCP-PCR） method. Results： ND3 and tRNA^Met＋Glu＋Ile gene fragments ofmtDNA from the tumors showed no variation in 27 endonuclease sites; D-loop ofmtDNA from Hca-F had an additional endonuclease sites of Hinf I in contrast to that of the inbred mouse. Deeply analyzed by PCR-SSCP, the D-loop ofmtDNA was found to possess mutations in 4 of 6 tumors. Conclusion： D-loop is the hot spot of tumor mtDNA mutation which can act as contributors to the carcinogenic

目的将在老鼠肿瘤查明线粒体 DNA (mtDNA ) 的变化并且调查在 mtDNA 和 carcinogenesis 的变化之间的关系。D 环， ND3 和从老鼠的六根肿瘤房间线的 mtDNA 的 tRNAMet+Glu+Ile 基因碎片的变化被 PCR 技术与限制分析的方法碎裂长度多型性分析(聚合酶链反应限制碎片长度多型性， PCR-RFLP ) 并且单个海滨符合构造多型性分析(SSCP-PCR ) 方法。从肿瘤的 mtDNA 的结果 ND3 和 tRNAMet+Glu+Ile 基因碎片没在 27 个 endonuclease 地点显示出变化；从 Hca-F 的 mtDNA 的 D 环有另外的 endonuclease Hinf 的地点我与生来的老鼠的相对照。深深地由 PCR-SSCP 分析了， mtDNA 的 D 环被发现在 6 个肿瘤中的 4 个拥有变化。结论 D 环是能充当贡献者到 carcinogenic 过程的肿瘤 mtDNA 变化的热点。