摘要
目的测定复方羊角片中阿魏酸的含量.方法采用高效液相色谱法.色谱柱为Kromasil C18色谱柱（4.6×250mm,5μm）,以甲醇-0.1%醋酸溶液（30∶70）为流动相;流速：1.0mL·min^-1,检测波长321nm.结果阿魏酸的进样量在0.08-0.28μg范围内呈良好的线性关系（r=0.9999）,平均加样回收率为100.3%,RSD为1.71%（n=6）.结论方法简便、准确,可用于复方羊角片中阿魏酸的含量测定.
OBJECTIVE To determine the ferulic acid in Fufangyangjiao Tablets.METHODS The HPLC analysis used Kromasil C18（4.6×250mm,5μm） as column,the mixture of methanol-0.1% acetic acid（30∶70） was used as mobile phase;The flow rate was 1.0ml·min^-1,the detection wavelength was 321nm.RESULTS The linear range of ferulic acid was 0.08-0.28μg（r=0.9999）,the average recovery was 100.3% with RSD 1.71%（n=6）.CONCLUSION The method was simple and accurate,it can be used for the determination of ferulic acid in Fufangyangjiao Tablets.
出处
《齐鲁药事》
2010年第12期721-722,共2页
qilu pharmaceutical affairs
