摘要
研究了灰毡毛忍冬次皂苷乙(MB)在体外对白血病细胞HL-60和结肠癌细胞LOVO增殖的抑制作用,并初步探讨其分子机制。采用MTT法检测MB的增殖抑制作用;利用RT2ProfilerTMPCR Array芯片实时定量PCR扩增肿瘤发生中84个关键基因。结果表明MB对两种肿瘤细胞生长均有抑制作用,且对HL-60效果更好。以HL-60作为细胞模型,总共发现差异基因20个,其中上调基因14个,下调基因6个,主要作用是阻滞细胞周期和降低细胞侵袭转移。
The cytotoxic effects and the molecular mechanism of Macranthoside B（MB） were investigated in this study.The cytotoxic effects of MB for LOVO and HL-60 cells were detected by MTT assay.Using RT2 ProfilerTM PCR Array,84 cancer-related genes were evaluated by a high-throughput quantitative real-time RT-PCR with or without MB treatment.Cell viability assay showed that MB inhibited cell growth of two cancer cell lines,especially in human acute promyelocytic leukemia HL-60 cells.As shown in microarray,a total of 20 differential genes were found,14 of which were upregulated and 6 were down-regulated.Their biologic functions demonstrate that MB may exhibit anti-tumor activity against HL-60 cells by arresting cell cycle and reducing cell metastasis.
出处
《天然产物研究与开发》
CAS
CSCD
2010年第5期765-768,811,共5页
Natural Product Research and Development
基金
江苏省自然科学基金(BK2008353)
江苏省药用植物研究开发中心开放基金(2008001)
