虫草素高产菌株的筛选及菌丝体中核苷类成分的分析

Screening of Cordyceps Strains with High Cordycepin Contents and Analysis of Nucleotides in Mycelia

用高效液相色谱对摇瓶培养获得的19个虫草属菌株的菌丝体及胞外液中核苷类成分进行了分析比较。结果表明,菌丝体中含有的核苷种类较多,且不同菌株菌丝体核苷类成分含量差异显著,而胞外液中的核苷主要为虫草素。同时发现虫草素主要分泌到培养基中,胞外液虫草素的含量可比菌丝体中高出40倍。通过筛选,获得一株虫草素高产菌株,培养液中虫草素含量可达366mg/L。

The nucleotides in submerged fermentation preparations of 19 Cordyceps strains were analyzed by using HPLC.The results showed that the nucleotides in mycelia were complicated and their contents in various strains were significant difference.Cordycepin was the main peak in liquid medium,and the yield of cordycepin in liquid was about 40 times higher than it in the mycelium.One strain with high cordycepin content was screened out,and the content of cordycepin in the liquid was up to 366 mg/L.