结核分枝杆菌宁夏分离株pstS1基因的扩增及生物信息学分析

Amplification and Bioinformatic Analysis of the pst S1 Gene from Ningxia Isolated Mycobacterium Tuberculosis

目的扩增编码结核分枝杆菌(宁夏分离株)38kDa蛋白的pst S1基因,运用生物信息学方法和软件预测其编码蛋白的生物学特性,为早期结核病的诊断以及新的抗结核药物的研制奠定基础。方法采用聚合酶链式反应(PCR),从结核分枝杆菌(宁夏分离株)基因组中扩增出pst S1基因,对其核苷酸序列进行测定;利用序列对比分析和蛋白质结构预测软件进行生物信息学分析。结果获得的pst S1基因的全长1112bp与预期大小基本一致,基因序列与GenBank公布的MTB标准株(ATCC27294)基因序列的核苷酸同源性为92.7%,初步预测出该蛋白的分子特征、抗原性和二级结构。结论成功扩增MTB(宁夏分离株)pst S1基因,通过生物信息学分析获得了该蛋白的信息特征,为进一步研究其生物学功能和免疫学活性奠定了基础。

Objective To amplify and analyze the pst S1 gene related to the protein secretion of Mycobacterium tuberculosis（Ningxia isolates） and to search it＇s role as new drug target or diagnosing Tuberculosis.Methods The pst S1 gene extracted from the genomic DNA of Mycobacterium tuberculosis（Ningxia isolates） was amplified by PCR and identification with nucleotide sequencing analysis,then its genome sequence and encoding proteins were analyzed by DNAstar.Results The pst S1 fragment was composed of 1112 base pairs and the nucleotide homology with type strain（ATCC27294）on the Gen Bank was 92.7%.The protein＇s bioinformatic features were anticipated.Conclusion The target gene had been amplified and it would provide the basis for further researching on its biological function.