摘要
以中华芦荟(Aloe vera L.var. chinensis)的叶片、顶芽、茎段和根为外植体进行离体快速繁殖技术体系研究,探讨了影响其快速繁殖的因素。结果显示,茎段为最适外植体,从株高为15 cm的芦荟植株中,切取长为1 cm的茎段,以正放置接种方式诱导不定芽效果最好,最佳诱导培养基为MS+6-BA2.0 mg/L+IAA2.0 mg/L,增殖系数为15.6。
The study aimed to construct the technology system of rapid propagation in Aloe vera L.var.Chinensis,used explants include leaf,shoot tip,stem and root.Results showed that,stem is protimum explant.To attain the best induced effect of dventitious bud,it must use the length of 1 cm cut from 15 cm aloe plant innoculated in culture media along the plant polarity.The best induced culture media is MS+6-BA 2.0 mg/L+IAA 2.0mg/L,propagate coefficient is 15.6.
出处
《惠州学院学报》
2010年第6期21-25,共5页
Journal of Huizhou University
关键词
中华芦荟
丛生芽
组织培养
快速繁殖
Aloe vera L.var. chinensis
tuft
tissue culture
rapid propagation