免疫法建立SD大鼠RAU模型

Establishment of Rats’ Recurrent Aphthous Ulcer with Immunology

目的:利用实验性大鼠口腔黏膜组织匀浆+弗氏佐剂注射方法,建立复发性口腔溃疡动物模型,为研究人类RAU的发病机制及治疗提供理论基础。方法:应用从实验性大鼠提取的口腔黏膜组织匀浆+弗氏佐剂,作为抗原免疫大鼠。将上述抗原注入大鼠脊柱双侧皮内,每周注射1次,共8周,观察大鼠口腔溃疡的发病及体重和摄食量的变化情况,并做溃疡的组织病理学、流式细胞仪测定外周血T淋巴细胞检测。结果:以口腔黏膜匀浆组织+弗氏佐剂作为抗原的实验大鼠都发生了RAU,溃疡模型建立过程中大鼠体重和摄食量均有显著统计学差异(P<0.05)。流式细胞仪测定外周血T淋巴细胞CD3+CD4+、CD4+/CD8+均低于对照组(P<0.05)。结论:利用大鼠口腔黏膜组织均浆作为抗原,可引起实验性大鼠发生RAU,其临床表现及组织学检查均与人类RAU相似。

Objective：To establish animal models of recurrent aphthous ulcer（RAU） . Methods：We established RAU models by injecting the mixture of homogenate tissue of rat＇s oral mucosal and complete Freund＇s adjuvant into 40SD rats. Antigen above added adjuvant was injected to intraderm adjacent the rats spine every week to observe the occurrence and cicatrization of oral ulcer in the experimental rats. The weight and food consumption of rats in each group was detected,and histopathology examination was performed with oral ulcer mucosa. The number of peripheral T-lymphocytes subsets and T-lymphocytes in circulating blood were tested by FCM. Results：All of the experimental rats developed oral ulcer. There was significant difference between control group and experimental group（P0.05） . In experimental groups,numbers of CD4＋ and the ratio of CD4＋ to CD8＋ in T-lymphocyte subsets were lower than those in control group（P0.05） . Conclusion：Our preliminary results indicated that this animal model could be used as model of RAU of human beings for further study on its pathogensis,and also suggested that the occurrence of RAU of rats may be related with food consumption,weight of rats and the ratio of CD4＋ to CD8＋ in T lymphocyte subsets. The ulcer was very similar to RAU in human beings clinically and pathologically.