雨生红球藻中豆甾醇的提取、纯化及表征

The Extraction, Purification and Characterization of the Stigmasterol from Haematococcus pluvialis

通过以二氯甲烷为溶剂对雨生红球藻粉进行浸提，再对提取液进行皂化的方法，初步得到雨生红球藻的有效成分。利用柱层析和重结晶技术，对有效成分粗品进行分离、纯化处理，得到了化合物I。利用FTr—IR，UV—Vis，Ms，^1H NMR ^13C NMR等谱学手段，对化合物I进行了表征，确定其为豆甾醇，且首次从雨生红球藻中被提取出来。

The activity component of Haematococcus pluvialis was separated by means of saponification after the dichloromethane extraction. The compound I was obtained by column chromatography and recrystallization. The compound I was characterized to determine it as stigmasterol by FT-IR, UV-Vis, MS, ^1H NMR, ^13C NMR, and it was the first time extracted from Haematococcus pluvialis.