高效液相色谱法测定三黄片中黄芩苷和盐酸小檗碱的含量

Quantitative Determination of Baicalin and Berberine Hydrochloride in Sanhuang Tablets by Reversed Phase High Performance Liquid Chromatography

建立了三黄片中黄芩苷和盐酸小檗碱的含量测定方法。采用高效液相色谱法。使用ODS（250mm×4．6mm，5μm）分析柱，流动相为甲醇-0．4％磷酸（40：60），流速为1mL／min，检测波长为275nm，柱温30℃．黄芩苷在0．1～1．0ug线性范围有良好的线性关系，盐酸小檗碱在0．3～3．0ug线性范围有良好的线性关系。黄芩苷和盐酸小檗碱的加样回收率分别为96．2％和95．4％，RSD分别为1．77％和1．93％（n=6）。此方法简便可行，快速准确，重现性好，可为药典完善三黄片的质量控制提供参考。

To establish a reversed-phase HPLC method for the quantitative detemination of Baicalin and Berberine Hydrochlorlde in Sanhuang Tablets. The experimental conditions were as follows：Phenomenex ODS column （250 mm × 4.6 mm,5 μm） ,mobile phase： 0.4% phosphoric acid（40： 60） ,flow rate ： I mL/min,detective wavelength：275 nm, column temperature：30℃. The calibration curve of Baicalin and Berberine Hydrochloride showed a good linearity . The test solution was stable in mobile phase within 24 h. The recovery rates were found to be for Baicalin and Berberine Hydrochloride were 96.2% and 95.4%, and RSD for them were 1.77% and 1.93% , respectively. The established method is simple feasible, fast, accurate, reproducible and reliable to operate and suitable for the quality control of Sanhuang Tablets.