摘要
茶条槭悬浮培养细胞经过10％浓硫酸．甲醇提取后，用HPLC法测定其没食子酸含量。色谱条件为：流动相为甲醇／水（4／6），流速0．5mL／min，Waters C18 MS柱，检测波长为270nm。实验测得的线性范围为20．0—100μg·mL^4（R=0．9996，n=5）。平均加样回收率为99．86％，RSD为0．09％。样品稳定性、实验的精密度及重复性三者的RSD均小于1．5％。表明本法是一种测定茶条械悬浮细胞中没食子酸含量的好方法。
A HPLC procedure was established to determin the content of gallic acid in suspension cells of Acer ginnala Maxim.. The separation was performed on Waters - C18 column with a mobile phase of methanol/water （4/6） and detected at wavelength of 270 nm. The results showed that the linearity range was 20.0-1000 μg · mL^-1（ R = 0. 9996, n = 5 ） and the average recovery of gallic acid was 99.86 % , RSD was 0.09 %. RSD values of sample stablity, precision and repetition were below 1.5%. All these results indicated that this preocedure works well.
出处
《天然产物研究与开发》
CAS
CSCD
2010年第B08期87-89,共3页
Natural Product Research and Development
基金
高等学校博士学科点专项科研基金（20060225010）
东北林业大学青年科研基金（07045）
关键词
茶条械
悬浮细胞
液相色谱
没食子酸
Acer ginnala Maxim.
suspension cell
HPLC
gallic acid
