摘要
分别用以DEAE．Sephadex A-50离子交换层析和Sepharose 4B-肝素钠亲和层析纯化猪凝血酶，对2种方法纯化结果进行比较。结果表明，离子交换层析纯化的凝血酶比活为1132IU／mg，纯化11．1倍，收率为45．3％；亲和层析的酶比活为1789IU／mg，纯化17．5倍，收率为58．3％；亲和层析纯化效果明显优于DEAE—SephadexA-50离子交换层析。
Porcine thrombin was purified by DEAE-Sephadex A 50 ion-exchange chromatography and Sepharose 4B-hep- arin affinity chromatography and the purification results were compared. The thrombin activity, purification fold and yield of purified thrombin by DEAE-Sephadex A 50 ion-exchange chromatography are 1132 IU/mg, 11.1 folds and 45.3% and those by Sepharose 4B-heparin affinity chromatography are 1789 IU/mg, 17.5 folds and 58.3%. The method of Sepharose 4B-heparin affinity chromatography is superior to that of DEAE-Sephadex A 50 ion-exchange chromatography.
出处
《天然产物研究与开发》
CAS
CSCD
2010年第B08期163-165,54,共4页
Natural Product Research and Development
基金
安徽省教育厅自然科学基金（KJ2010A328）
关键词
凝血酶
离子交换层析
亲和层析
分离纯化
thrombin
ion-exchange chromatography
affinity chromatography
purification
