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水稻强分蘖基因MT1的克隆、转化及鉴定

Cloning,Transforming and Identifying of a Multiple-tillering Gene MT1 in Japonica Rice(Oryza sativa L.)
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摘要 在本研究中,从覆盖MT1基因的水稻BAC克隆OSJNBa0014K08中切出MT1基因序列,将其转化到水稻强分蘖突变体mt1-1中,并对转基因后代进行鉴定,结果发现,转基因植株的分蘖性状当代就恢复了正常,这就说明该基因就是控制水稻强分蘖性状的MT1基因。 In this research,to transform a DNA fragment of full length MT1 gene that was found in a BAC contig,AP003376,to mt1-1 mutant,the tillering character of the transplants was recovered.It indicated that this gene was the actual gene MT1 which controlled the strong branching of rice.
作者 史俊
机构地区 江苏农林职业技术学院
出处 《安徽农学通报》 2010年第24期16-18,共3页 Anhui Agricultural Science Bulletin
关键词 水稻 强分蘖 转基因 MT1 Rice（Oryza sativa L.） Multiple-tillering gene Transgene MT1
分类号 F511.032 [经济管理—产业经济]
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  • 1SELKER E U.Gene silencing: Repeats that count [J].Cell,1999,65(3):205-209.
  • 2COGONI C Macino G.Gene silencing in Neurospora crassa requires a protein homologous to RNA polymerase[J].Nature,1999,399(6732):166-169.
  • 3DALMAY T Hamilton A Rudd S et al.An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus[J].Cell,2000,101:543-553.
  • 4NAN X, Ng H H, Johnson C A, et al. Transcriptional repression by the methyl CpG-binding protein MeCP2 involves a histone deacetylase complex[J].Nature, 1998,393 : 386-389.
  • 5MATZKE A J M, Neuhuber F, Park Y D, et al.Homology-dependent gene silencing in transgenic plants:epistatic silencing loci contain multiple copies of methylated transgenes [J]. MGG, 1994,244:219-229.
  • 6WASSENEGGER M. RNA-directed de novo methylation of genomic sequences in plants[J]. Cell, 1994,76:567-576.
  • 7CARROLYN N, Lemieux C, Jorgensen R. Introduction of a chimetic chalcone syntase gene into petunia results in reversible co-suppression of homologous genes in trans[J]. Plant Cell, 1990,23 : 279-289.
  • 8MEYER P, Heidmann I. Epigenetic variants of a transgenic Petunia line show hypermethy-lation in transgene DNA: an indication for specific recognition of foreign DNA in transgenic plant [J]. Mol Gen Gengt, 1994,243: 390-399.
  • 9LEBEL E G, Masson J,Bogucki A, et al. Transposable elements as plant transformation vectors for long stretched foreign DNA[J]. There Appl Genet,1995,91:899-906.
  • 10SONGSTD D D, Somers D A, Griesbach R J. Advances in alternative DNA delivery techniques [J].Plant Cell Tiss Org Cult, 1995,40:1-15.

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安徽农学通报
2010年 第24期

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