化学药剂防治水稻纹枯病的新筛选方法──蚕豆叶片法

A Simple Bioassay Method of New Fungicide against RiceSheath Blight-Broad Beau Leaf Method

本研究以建立简便、快速的杀菌剂筛选方法为目标，在前人研究工作的基础上，建立了适用于水稻纹枯病（Ｐｅｌｌｉｃｕｌａｒｉａｓａｓａｋｉｉ）杀菌剂生物测定模式──蚕豆叶片法。试验表明，该方法具有测定结果准确、方法简便、取材容易等优点。采用该方法对Ｐｅｎｃｙｃｕｒｏｎ、井岗霉素进行试验，获得了与国内外报导一致的结果，与水稻植株法的防治效果具有高的相关性。通过对Ｐ．ｓａｓａｋｉｉ菌龄的研究，认为致病菌的菌龄对侵染有明显影响，愈是新鲜菌丝侵染力愈强。

The aim of this study was to establish one easy and rapid fungicidal screening method.A“broad beau leaf method” appropriated for fungicidal bioassay of rice sheath blight was proposed. The experiment showed that the method was simple to prepare the meterials and masterthe technique. Antibiotic activity and action way of Pencycuron and Validmycin assayed by thismethod were the same as those reported in the literatures. Similar results were obtained by usingeither broad beau leaf method or seedling method. The experimental results justified that the ageof pathogenic fungi had a great influence on infectivity: the younger the hypha of P. sasakii, thestronger was its infectivity.