摘要
旨在探讨三聚氰胺及其同系物三聚氰酸对肾脏组织的损伤及其损伤机理,为防制其对动物泌尿器官的危害提供理论依据。120只雄性SD小鼠随机分为6组,每组20只,体质量20～30g。对照组蒸馏水灌胃,染毒组采取隔天灌胃方式,A组三聚氰胺50mg·kg-1体质量,B组三聚氰酸50mg·kg-1体质量,C、D、E组三聚氰胺与三聚氰酸混合物(三聚氰胺+三聚氰酸等量混合)高、中、低剂量分别按50、10、2mg·kg-1体质量灌胃,持续30d。测定血清尿素氮(BUN)、肌酐(CRE),肾脏组织匀浆中超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量,观察肾组织形态学,TUNEL法检测肾脏组织细胞凋亡,RT-PCR法检测肾脏组织Bcl-2、Bax、Cyt-c基因的相对表达以及免疫组化法检测相关蛋白的表达。三聚氰胺与三聚氰酸混合物能引起明显的肾脏组织细胞损伤,呈剂量相关性,表现为BUN和CRE升高,SOD活性降低,MDA含量升高,组织形态学可见明显的肾小管损害;混合组凋亡率明显增高,其中混合高剂量组与对照组相比,差异极显著(P<0.01),Bax、Cyt-c表达均高于对照组,而Bcl-2则低于对照组。三聚氰胺与及其与三聚氰酸混合物可以通过脂质过氧化和线粒体通路介导凋亡共同作用引起肾损伤。
The aim of the study was to investigate the effects of melamine and cyanuric acid on renal injury.120 male SD mice were randomly divided into six groups：control group,melamine group（50 mg·kg-1bw）,cyanuric acid group（50 mg·kg-1bw）,melamine ＋ cyanuric acid group（equal amount of melamine and cyanuric acid mixed） at the dose of 50,10 and 2 mg·kg-1bw,respectively.Mice were administrated drugs for 30 days at 48 h intervals,and the follow researches were taken：determination of the level of blood urea nitrogen（BUN） and creatinine（CRE） in serum,activity of superoxide dismutase（SOD） and content of malondialdehyde（MDA） in kidney homogenization,observation of renal tissue morphological;apoptosis of renal cell by TUNEL,expression of Bcl-2,Bax and Cyt-c gene by RT-PCR,and expression of the related proteins by immunohistochemistry.The results showed that the mixture of melamine and cyanuric acid could significantly induce renal injury in a dose-dependent manner.Such as the level of BUN and CRE in serum were increased,the activity of SOD was decresed and the content of MDA was enhanced,and renal tubular injury were obviously observed,apoptotic rate of renal cell in melamine ＋ cyanuric acid group at the medium and high doseage was markedly higher than that in the control group（P0.01）.At the same time,some changes of genes expression were also found,a markedly increase in the expression of Bax and Cyt-c gene and a markedly decrease in the expression of Bcl-2 gene were observed.Renal injury melamine-and cyanuric acid-induced caused by lipid peroxidation and mitochondria-mediated apoptotsis.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第12期1614-1621,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
湖南省教育厅重点项目(03A02000)
