超声乳化术对白内障模型犬的治疗研究

Therapeutics Research of Phacoemulsification in Canine Cataract Models

犬白内障是兽医临床的常见疾病,氧化损伤是其重要发病机制,目前较为有效的治疗方法是超声乳化术。鉴于国内对犬白内障疾病模型与治疗研究报道甚少,作者旨在对此进行探索和实践。选取健康成年犬15只,13只为模型组,2只为对照组。用H2O2稀释液造模,分别对其晶状体形态结构进行光镜与透射电镜观察;剩余10例左眼采用超声乳化术治疗,右眼作为对照;通过分析晶状体混浊比值、眼内压与每分钟泪液量来评估建模方法和手术疗效。结果表明:犬白内障模型成功建立,形态学上显示晶状体上皮细胞空泡化,线粒体空泡化;使用超声乳化术治疗白内障模型犬可使切口愈合,视力恢复良好。本研究证实晶状体混浊比值是反映犬白内障模型建立成功与否的重要指标之一;超声乳化术可治疗犬白内障,适宜临床推广应用。

Canine cataract is a common disease in veterinary clinic,and oxidative damage is an important pathogenesis of the cases.Nowadays more effective therapy is phacoemulsification.This study was conducted to establish canine cataract model by H2O2 and explore therapeutics.Fifteen adult dogs were selected,13 for modeling and 2 for control.Phacoemulsification was used to cure the left eyes in model dogs.We assessed the method about the model establishment and the curative effect of phacoemulsification by lens opacity ratio,intraocular pressure and Schirmer tear test.We investigated the morphology characteristics of lens by light microscope and transmission electron microscope.Results showed that 13 canine cataract models have established by detecting lens and mitochondrion vacuolization.After phacoemulsification,the models showed the incision healing and eyesight recovering well.The index of lens opacities ratio was proved to be more sensitive marker for the canine cataract modeling.Phacoemulsification could be used to cure canine cataract cases and it would be adopt in veterinary clinic.