针刺对局灶性脑缺血大鼠缺血脑组织及血清IL-6的影响

Influence of acupuncture on IL-6 in ischemic brain tissue and serum in rats with focal cerebral ischemia

[目的]观察针刺对局灶性脑缺血大鼠脑组织及血清白介素-6(IL-6)含量的影响。[方法]以热凝法阻断一侧大脑中动脉造成大鼠局灶性脑缺血模型,采用放免法测定大鼠脑组织及血清IL-6含量。[结果]脑缺血后3、6、12、24 h缺血组脑组织IL-6含量与正常组比较均显著增高(P<0.01),针刺后3、6、12 h脑组织中IL-6含量较同时段缺血组显著降低(P<0.01),并明显低于正常对照组(P<0.01)。脑缺血后血清中IL-6含量3 h急剧升高,3、6、12 h段缺血组血清IL-6含量与同时段假手术及正常组比较显著升高(P<0.01),治疗后3、6、12 h针刺组血清IL-6含量较同时段缺血组显著降低(P<0.01),24及48 h针刺组血清IL-6含量明显升高,与正常组及假手术组比较均有显著差异(P<0.01)。[结论]通过对IL-6的调节,发挥其抗炎、神经保护作用,促进受损神经元修复,可能针刺治疗缺血性脑卒中的作用机制之一。

[Objective]To observe the influence of acupuncture on the expression of IL-6 in brain tissue and serum in rats with focal cerebral ischemia.[Method] Focal cerebral ischemia in rat was established by obstructing one side middle cerebral artery with thermocoagulation method,and the expression of IL-6 in brain tissue and serum was measured with radio-immunologic method.[Result] Compared with the normal group,the expression of IL-6 in brain tissue and serum in the ischemia group at 3,6,12,24 hours after cerebral ischemia was all significantly increased（P0.01）.Compared with the ischemia group at the same time periods,the IL-6 expression in brain tissue in the acupuncture group at 3,6,12 hours after acupuncture treatment was significantly reduced（P0.01）,and was obviously lower than that in normal group（P0.01）.After cerebral ischemia,the serum IL-6 expression sharply increased after three hours.Compared with the sham operation group and the normal group at the same time periods,the serum IL-6 expression in the ischemia group at 3,6,12 hours was significantly increased（P0.01）.Compared with the ischemia group at the same time periods,the serum IL-6 content in the acupuncture group at 3,6,12 hours after treatment was significantly reduced（P0.01）.The serum IL-6 expression in the acupuncture group at 24 and 48 hours was obviously increased,and compared with the normal group and the sham operation group it had significant difference.（P0.01）.[Conclusion]Through regulating IL-6,the acupuncture can exert its anti-inflammatory and neuro-protective effect,and can promote the recovery of the injured neurons.That may be one of the effect mechanisms of acupuncture for treating ischemic cerebral apoplexy.