高糖高脂诱导脐静脉内皮细胞凋亡及对线粒体膜电位的影响

The effect of high glucose and fat on apoptosis of endothelial cell in umbilical vein and mitochondria membrane potential

[目的]明确糖基化终末产物(AGEs)和氧化低密度脂蛋白(ox-LDL)联合诱导内皮细胞凋亡及其作用机制。[方法]以AGEs和ox-LDL联合作用人脐静脉内皮细胞24 h,hochest33258染色后观察凋亡,Rhodamin123染色后流式细胞术检测线粒体膜电位。[结果]研究表明AGEs和ox-LDL共同作用可增加内皮细胞的凋亡率,降低内皮细胞线粒体膜电位。[结论]提示高糖高脂促进内皮细胞凋亡,可能和降低线粒体膜电位有关。

[Objective]To study the mechanism of apoptosis of human umbilical vein endothelial cell（HUVEC） induced by AGEs and ox-LDL.[Methods]HUVECs were incubated at some concentration of AGEs and ox-LDL for 24 h.The apoptosis was observed by Hochest 33258 staining.The mitochondria membrane potential（MMP） was analyzed by flowcytometry after Rhodamine-123.[Results]AGEs and ox-LDL could increase apoptosis significantly and decrease mitochondria membrane potential of the cultured HUVECs significantly also（P0.05）.[Conclusion]The results indicated that AGEs and ox-LDL could promote endothelial cell apoptosis,the mechanism in which may be related to decreasing of mitochondria membrane potential.