氨基胍与川芎嗪对糖尿病大鼠胰岛B细胞的保护作用

The protective effect of aminoguanidine and ligustrazine on islet B cells in diabetic rats

目的探讨氨基胍与川芎嗪对糖尿病大鼠胰岛B细胞的保护作用。方法应用链尿佐菌素(STZ)制作糖尿病大鼠模型,分为正常对照组、糖尿病未治疗组、川芎嗪治疗组、氨基胍治疗组和氨基胍联合川芎嗪治疗组,于第12周取胰尾组织行免疫组织化学观察。结果氨基胍治疗组、氨基胍和川芎嗪联合治疗组中胰岛B细胞免疫阳性染色的面积及细胞数量明显增加,与糖尿病未治疗组比较差异有显著意义(P<0.05);但川芎嗪治疗组与糖尿病未治疗组比较差异无显著意义(P>0.05)。结论氨基胍和川芎嗪对糖尿病胰岛B细胞具有保护作用,其中氨基胍起主要作用,两者相互协同。

Objective To study the effect of aminoguanidine and ligustrazine on islet B cells in diabetic rats.Methods Diabetes was induced by streptozotocin（STZ）.The experimental rats were divided into 5 groups： normal control group,diabetic group without treatment,diabetic group treated with tetromethylpragine,diabetic group treated with aminoguanidine,and diabetic group treated with tetromethylpragine and aminoguanidine.The tail of pancreas was observed by immunohistochemical staining in the 12th week.Results The positive stain area of immunity reaction and the number of islet cell were obviously increased both in the diabetic group treated with aminoguanidine（P0.05）,and in diabetic group treated with tetromethylpragine and aminoguanidine,and showed significant differences compared with the diabetic group without treatment.But there were no significant differences between diabetic group treated with tetromethylpragine and the diabetic group without treatment（P0.05）.Conclusion Aminoguanidine leads a main role in the protective effect of aminoguanidine and ligustrazine on islet B cells in diabetic rats.