黄芪多糖与Ⅰ型胶原协同促血管新生的细胞学研究

The Synergy Effects of Astragalus Polysaccharide and Collagen Ⅰ to Promote Angiogenesis on cellular level

目的观察黄芪多糖载入Ⅰ型胶原促血管新生作用,探讨黄芪多糖与Ⅰ型胶原的协同作用。方法通过人脐静脉血管内皮细胞(HUVEC)增殖实验测定不同浓度黄芪多糖和胶原对细胞的增殖作用,并测量细胞迁移率;Western Blot方法验证血管生成素-1(Ang-1)、整合素蛋白αvβ3、血管内皮生长因子(VEGF)的表达。结果黄芪多糖浓度20μg/mL时促进HU-VEC生长作用最明显,此后随着浓度的升高,促生长的作用逐渐降低,但均显著高于对照组(P<0.01);而黄芪和胶原合用,对细胞的促生长有协同作用,显著高于20μg/mL胶原组(P<0.05)。20μg/mL黄芪多糖+20μg/mL胶原时HUVEC迁移能力最强;Western Blot结果提示Ang-1、VEGF和integrinαvβ3的表达随着黄芪和胶原浓度的增加而升高;20μg/mL黄芪多糖+胶原组达到最高。结论黄芪多糖能促进HUVEC的增殖和迁移,并上调Ang-1、VEGF和Integrinαvβ3的表达,具有促进血管新生的作用,且黄芪多糖和胶原具有协同作用。

Objective To explore the synergistic effect of astragalus polysaccharide （APS） and collagen type Ⅰ andits molecular mechanism.Methods The growth promoting effects of APS and collagenI with different concentration on human umbilical vein endothelial cells （HUVEC） was assessed by MTT.The Transwell little room was used to examine the migration capability. Western blot was employed to detect the expression levels of angiogenin-1 （Ang-1） ,integrin αvβ3 and vascular endothelial growth factor（VEGF）.Results APS with the concentration of 20μg/mL had the most significant growth promoting effect on HUVEC. Then,increasing the concentration would lead to decease of the effect . But both APS with the concentration of 20μg/mL and higher had a significant growth promoting effects compared with the control group （P0.01） .The synergy effects on growth promoting were observed whencollagenI was administrated with a stragalus polysaccharide, which was more effective than APS alone at the concentration of 20μg/mL （P0.05） .The expressions of Ang-1,VEGF and integrin αvβ3 increased as the increasing of the concentration of APS and collagen Ⅰ,which got to the peak when the collagen Ⅰ and 20μg/ml APS was used.Conclusions APS can promote the proliferation and migration of HUVEC,and increases the expressions of Ang-1,VEGF and the integrin αvβ3.And the synergistic effect was discovered when APS and collagen Ⅰ were administrated together.