影响花叶开唇兰原球茎与丛生芽形态建成、生根与移栽因素的试验研究

Factors Influencing Shape Building of Protocorm and Cespitose Bud, Rooting and Transplanting of Anoectochilus roxburghii

通过对比试验和正交试验研究花叶开唇兰原球茎与丛生芽形成、生根与移栽的影响因素。结果表明， 在 Ｂ Ａ 诱导下， 不带顶芽的外植体比带顶芽的外植体容易形成原球茎和丛生芽， 但诱导率高低与 Ｂ Ａ 浓度关系不太； Ｎ Ａ Ａ 是影响组培苗生根的关键因子， Ｉ Ｂ Ａ 和大量元素用量对生根率影响不大， 但添加 Ｉ Ｂ Ａ 和使用全量 Ｍ Ｓ有利于生根苗发根和壮苗； 保持较高湿度及适宜的温度 （１８℃～２３℃） 并适当遮荫， 可以保证移栽的生根苗获得很高的成活率， 但植株生长极缓慢。

The factors influening shape building of protocorm and cespitose bud,rooting and transplanting of Anoectochilus roxburghii were researched by comparative test and orthogonal test.The results demonstrated that under the inducement of BA,the explants without terminal bud generating protocorms and cespitose buds were more eazier than the one with terminal bud,and the rate of inducement has little to do with the consistency of BA.The key factor influencing the rooting of tissue culture seedlings is NAA.The content of IBA and macronutrient have littile influence on the rooting rate,but adding IBA to the medium and using total MS may heighten the stalks,strenghten the seedlings and increase the number of roots.Transplanting the rooted seedlings, at high moisture,proper temperature(18℃~23℃) and shaded surroundings,had higher survival rate,but the plants grew quite slowly.