脑出血患者脂蛋白脂酶Ser447Ter基因多态性的研究

The relationship between the gene polymorphism in lipoprotein lipase and cerebral hemorrhage

目的探讨脂蛋白脂酶(LPL)Ser447Ter基因多态性与脑出血关系。方法应用聚合酶链式反应-限制性片段长度多态性技术(PCR-RFLP)对313例脑出血患者和351例正常对照者脂蛋白脂酶(LPL)Ser447Ter基因多态性进行研究。结果在脑出血组中,LPLSer447Ter3种基因型频率分别为CC85.6%、CG14.1%和GG0.3%。LPLSer447Ter基因型和等位基因频率的分布在脑出血组与对照组之间无显著性差异(P>0.05);而LPLSer447TerCG+GG基因型携带者甘油三酯(TG)、载脂蛋白B(ApoB)和脂蛋白(a)[LP(a)]水平低于GG基因型携带者(P<0.05)。结论研究未发现LPLSer447Ter基因多态性与脑出血存在相关关系。

Objective To investigate the correlation between the lipoprotein lipase Ser 447Ter polymorphism and cerebral hemorrhage. Methods The genotype and allele frequencies of LPL Ser 447Ter polymorphism were determined in 313 cases with cerebral hemorrhage and 351 normal controls by PCR-RFLP. The serum lipid and lipoprotein levels were measured by routine method. Results The LPL Ser 447Ter genotype distribution in the cerebral hemorrhage group studied was CC 85.6% , CG 14.1% and GG 0.3% ,respectively. There was no significant difference in LPL Ser 447Ter allele and genotype distribution between the cerebral hemorrhage group and the control group （P0.05）. But the level of serum triglyceride, ApoB and LP（a） in LPL CG or GG carriers was lower than that in LPL CC carriers （P0.05）. Conclusions These results suggest that the LPL Ser 447Ter gene polymorphism is not associated with cerebral hemorrhage.