Wolbachia在Drosophila auraria复合种和Drosophila simulans中的感染

Wolbachia Infection in Drosophila auraria Species Complex and Drosophila simulans

用 Ｐ Ｃ Ｒ 方法检测了多种果蝇中共生菌 Ｗ ｏｌｂａｃｈｉａ 的感染，发现 Ｄｒｏｓｏｐｈｉｌａ ａｕｒａｒｉａ 复合种以及采集于北京地区的 Ｄｒｏｓｏｐｈｉｌａ ｓｉｍ ｕｌａｎｓ为 Ｗｏｌｂａｃｈｉａ 所感染， Ｒ Ｆ Ｌ Ｐ分析证实为单一感染．克隆了编码 Ｗ ｏｌｂａｃｈｉａ 外膜蛋白质的 ｗ ｓｐ 基因并进行了序列测定．同时比较了 Ｄｒｏｓｏｐｈｉｌａ ａｕｒａｒｉａ 复合种内 ４ 个种和采集于北京及美国加州的 Ｄｒｏｓｏｐｈｉｌａ ｓｉｍ ｕｌａｎｓ 的线粒体的细胞色素氧化酶 ２ 亚基基因的部分序列． 进而讨论了 Ｗｏｌｂａｃｈｉａ 对 Ｄｒｏｓｏｐｈｉｌａ ａｕｒａｒｉａ 复合种成员之间进化关系的影响．

The existence of Wolbachia in Drosophila has been detected by using PCR. Drosophila auraria species complex and Drosophila simulans collected in Beijing are found to be infected with Wolbachia . The infection is proved to be caused by a single strain of Wolbachia by RFLP analysis. The fragments of wsp gene from these fruitflies,which encodes a Wolbachia outer surface protein,were cloned and sequenced. Meanwhile,the mitochondria COⅡ gene fragments have also been cloned,sequenced and compared with each other. The influence of Wolbachia on members of Drosophila auraria species complex has been discussed.