水稻染色体微切割及4号染色体酶解片段的扩增

Microdissection and Amplification of Rice Chromosome No.4

通过细胞分裂的同步化、低渗、酶消化、瞬间固定和涂片等制片技术，快速制备水稻根尖体细胞染色体标本．该方法制备出的染色体标本，杂质少、染色体分散程度好，满足了染色体微切割用片的要求．染色体微切割和ＰＣＲ扩增技术，获得了水稻第４ 号染色体ＤＮＡ 文库．经ＦＩＳＨ法的初步鉴定，所获得的ＤＮＡ 确来自水稻细胞核．这为植物特定染色体（或相应区段）的基因克隆提供方便．

By the treatments of synchronization of meristematic cell division in root tips,hypotonic method,transient fixation, enzyme digestion and smearing technique, well spreaded and low contaminated rice chromosomes were quickly prepared and fitted for chromosome microdissection. No.4 chromosomal DNA library was constructed by means of chromosome microdissection and PCR technique. It was primarily confirmed by FISH technique that the DNA library was really from rice cell nuclei. It will be helpful for cloning plant genes located in specific chromosome or related region.