猪传染性胃肠炎病毒S蛋白的二级结构和B细胞抗原表位的预测

Prediction of Secondary Structure and B cell Epitopes of TGEV Spike Protein

以TGEV(猪传染性胃肠炎病毒)基因组序列为基础,运用Garnier-Robson、Chou-Fasman和Karplus-Schulz方法预测其S蛋白的二级结构,运用Kyte-Doolittle方法对S蛋白的亲水性进行分析,并分别运用Jameson-Wolf方法和Emini方法预测了其抗原指数和表面可及`性,最后结合吴玉章的方法综合分析预测了其B细胞抗原表位。结果显示,α螺旋数量少且分散,β折叠占据面积较大,柔性区域主要集中在N端第22～30、45～76、100～172、239～301、348～419、451～519、541～633、645～720、746～796、822～887、921～986、1052～1201、1239～1384、1434～1445区段。S蛋白可能的B细胞抗原位点是N端第20～32、44～54、69～78、97～106、635～655、781～803、949～994、1307～1328、1346～1362、1432～1448区段,这些区段内部或附近都有柔性区域存在,可作为S蛋白的抗原优势表位。

The secondary structure of TGEV spike protein was predicted with the methods of Garnier-Robson,Chou-Fasman and Karplus-Schulz.Its hydrophilicity,surface probability plot and antigenic index were obtained by the methods of Kyte-Doolittle,Emini and Jameson-Wolf respectively.Then the method of WU Yu-zhang was integrated to analyze the B cell epitopes.The results showed that a few α helix regions were distributed in the protein.Β sheet regions occupied a large area.The flexible regions located intensively at the N terminal regions of 22～30,45～76,100～172,239～301,348～419,451～519,541～633,645～720,746～796,822～887,921～986,1052～1201,1239～1384 and 1434～1445.The B cell epitopes of TGEV spike protein were probably located at or adjacent to the N terminal regions of 20～32,44～54,69～78,97～106,635～655,781～803,949～994,1307～1328,1346～1362 and 1432～1448.Some flexible regions were in these regions or hereabout,so they could be used as the preponderant epitopes of spike protein.